IVF Basics HTF (HEPES) Ready-to-use

IVF Basics® HTF and HTF HEPES with 0.4% HSA are ready-to-use media and have been developed for washing, handling, conditioning and culturing of human gametes and embryos.

IVF Basics® HTF 0.4% HSA should be pre-equilibrated overnight or at least for a minimum of 4 hours before use at 37ºC and 5% CO2.

IVF Basics® HTF HEPES 0.4% HSA contains HEPES, no CO2 incubation is required.

Features & Benefits

Ready-to-use
Contains Gentamicin
Bicarbonate buffered
HEPES buffered to maintain a stable pH during procedures outside a CO2 incubator (HTF HEPES w/ HSA)
Phenol red for visual pH control (HTF w/ HSA)
Storage at 2oC to 8oC
500ml bottles
1,5 year shelf life

Product Codes

Product

Product code

Size

IVF Basics HTF 0.4% HSA

HTF-B-0,4A-10

10 ml

IVF Basics HTF 0.4% HSA

HTF-B-0,4A-30

30 ml

IVF Basics HTF 0.4% HSA

HTF-B-0,4A-60

60 ml

IVF Basics HTF 0.4% HSA

HTF-B-0,4A-120

120 ml

IVF Basics HTF 0.4% HSA

HTF-B-0,4A-250

250 ml

IVF Basics HTF 0.4% HSA

HTF-B-0,4A-500

500 ml

IVF Basics HTF HEPES 0.4% HSA

HTF-H-0,4A-10

10 ml

IVF Basics HTF HEPES 0.4% HSA

HTF-H-0,4A-30

30 ml

IVF Basics HTF HEPES 0.4% HSA

HTF-H-0,4A-60

60 ml

IVF Basics HTF HEPES 0.4% HSA

HTF-H-0,4A-120

120 ml

IVF Basics HTF HEPES 0.4% HSA

HTF-H-0,4A-250

250 ml

IVF Basics HTF HEPES 0.4% HSA

HTF-H-0,4A-500

500 ml

Composition & Quality Control

IVF Basics® HTF and IVF Basics® HTF HEPES with 0.4% HSA consist of a balanced physiologic salt solution, supplemented with carbohydrate energy sources such as glucose, pyruvate and lactate, and 10mg/liter gentamicin sulphate (medicinal substance). IVF Basics® HTF 0.4% HSA is bicarbonate buffered while IVF Basics® HTF HEPES 0.4% HSA additionally contains HEPES.

IVF Basics® HTF 0.4% HSA and IVF Basics® HTF HEPES 0.4% HSA are presupplemented with 4g/liter HSA (medicinal substance derived from human blood plasma) and are ready-to-use.

pH:

7,20 – 7,60 (At 5% CO2 for HTF 0.4% HSA)

Osmolality:

270 – 290 mOsm/kg

Sterility test by the
current pH. Eur.
2.6.1 / USP <71>:

No growth

Endotoxin:

< 0,25 EU/ml

Mouse Embryo Assay:

≥ 80%

Human sperm survival assay (HSSA)(24h exposure):

≥ 80%

Support Documents

Instructions For Use

IFU: Europe
IFU: UA

Safety Data Sheet

MSDS

Summary of Safety and Clinical Performance

SSCP

Certificate of Analysis

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