IVF Basics HTF

IVF Basics^® HTF has been developed for washing, handling, conditioning and culturing of human gametes and embryos.
IVF Basics^® HTF should be pre-equilibrated overnight or at least for a minimum of 4 hours before use at 37ºC and 5% CO₂.

Features & Benefits

Contains Gentamicin
Bicarbonate buffered
Phenol red for visual pH control
Designed for use in a CO₂ incubator in a humified atmosphere
Storage at 2^oC to 8^oC
500ml bottles
1,5 year shelf life

Product Codes

Product	Product code	Size
IVF Basics HTF	HTF-B-10	10 ml
IVF Basics HTF	HTF-B-30	30 ml
IVF Basics HTF	HTF-B-60	60 ml
IVF Basics HTF	HTF-B-120	120 ml
IVF Basics HTF	HTF-B-250	250 ml
IVF Basics HTF	HTF-B-500	500 ml

Composition & Quality Control

IVF Basics^® HTF consists of a balanced physiologic salt solution, supplemented with carbohydrate energy sources such as glucose, pyruvate and lactate, and 10mg/liter gentamicin sulphate (medicinal substance). IVF Basics^® HTF is bicarbonate buffered.

It is strongly suggested to supplement IVF Basics^® HTF with 4g/liter HSA (medicinal substance derived from human blood plasma) before use.

pH:	7,20 – 7,60 (At 5% CO₂)
Osmolality:	270 – 290 mOsm/kg
Sterility test by the current pH. Eur. 2.6.1 / USP <71>:	No growth
Endotoxin:	< 0,25 EU/ml
Mouse Embryo Assay:	≥ 80%
Human sperm survival assay (HSSA)(24h exposure):	≥ 80%