HTF HEPES - IVF Basics®
IVF Basics® HTF HEPES has been developed for washing, handling, conditioning and culturing of human gametes and embryos.
IVF Basics® HTF HEPES contains HEPES, no CO2 incubation is required.
Features & Benefits:
Contains Gentamicin
HEPES buffered to maintain a stable pH during procedures outside a CO2 incubator
Storage at 2oC to 8oC
500ml bottle
1,5 year shelf life
Composition:
IVF Basics® HTF HEPES consists of a balanced physiologic salt solution, supplemented with carbohydrate energy sources such as glucose, pyruvate and lactate, and 10mg/liter gentamicin sulphate (medicinal substance). IVF Basics® HTF HEPES is bicarbonate buffered and additionally contains HEPES.
It is strongly suggested to supplement IVF Basics® HTF HEPES with 4g/liter HSA (medicinal substance derived from human blood plasma) before use.
Quality control:
- pH: 7,20 – 7,60
- Osmolality: 270 - 290 mOsm/kg
- Sterility test by the No growth
current pH. Eur.
2.6.1 / USP <71>
- Endotoxin: < 0,25 EU/ml
- Mouse Embryo ≥ 80%
Assay:
- Human sperm ≥ 80%
survival assay (HSSA)
(24h exposure):
- Certificate of Analysis available upon request.