SpermWash® Medium
SpermWash® is intended for in vitro procedures involving human gametes (sperm and oocytes), including washing of gametes, sperm swim-up procedures, intra-uterine insemination (IUI) of the spermatozoa and sperm injection during intracytoplasmic sperm injection (ICSI). SpermWash® can also be used for human embryo washing and holding, and for embryo transfer (ET) in the uterine cavity.
Features & Benefits:
Ready-to-use
1,5 years shelf life
Due to HEPES buffer no need for CO2 incubation
Sterilized by using aseptic processing techniques
Composition:
SpermWash® is a ready-to-use HEPES buffered medium which also contains phenol red, bicarbonate, physiologic salts, glucose, lactate, pyruvate and Human Serum Albumin (4.0 g/l; medicinal substance derived from human blood plasma) and gentamicin (10 mg/l, medicinal substance).
Quality control:
- pH: 7,30 – 7,90 (Release criteria 7,30 – 7,60 )
- Osmolality: 270 - 290 mOsm/kg
- Sterility test by the No growth
current pH. Eur.
2.6.1 / USP <71>
- Endotoxin: < 0,25 EU/ml
- Mouse Embryo ≥ 80%
Assay:
- Human Serum 3,2 - 4,8 g/l
Albumin
- Human sperm ≥ 80%
survival assay (HSSA)
(24h exposure):
- Certificate of Analysis available upon request.